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The grant program aims to support the establishment of a Newborn Screening (NBS) Center of Excellence. This center will focus on: developing infrastructure to implement existing, advanced technologies to expand NBS system capacity; using advanced technologies as second and third tier testing platforms to optimize screening performance; and incorporating bioinformatics to analyze complex biochemical and molecular data. Over a four-year project period, the recipient will pilot methods to enhance NBS practices in public health laboratories, collaborate and share findings with the broader NBS community, and partner with CDC experts on data harmonization and modernization. This initiative targets to equip NBS programs with the ability to adapt to advanced technologies and address increasing demands. The program is specifically open to applicants that represent or are in administrative partnership with established state or territorial newborn screening programs in the United States, with the capacity to implement and sustain these advancements.